PROTEIN SENSITIZATION OR ANAPHYLAXIS 275 



one hour. The supernatant fluid is then removed and is 

 known as the organ extract. Egg-white and horse serum 

 are diluted with physiological salt solution until 0.1 c.c. 

 contains the amount of protein desired in the individual 

 experiment, but in case more than 10 mg. of protein is 

 used, a multiple of 0.1 c.c. constitutes the solvent. These 

 solutions are freshly prepared for each experiment, and 

 everything is done aseptically. The volume of serum or 

 organ extract used is 5 c.c., and the amount injected into 

 the heart of the animal is, unless otherwise noted, 4 c.c. 

 Further details will appear in the record of the experiments. 



1. One milligram of egg-white incubated for thirty 

 minutes in 5 c.c. of the serum or organ extracts of a normal, 

 unsensitized guinea-pig is without marked effect when 

 injected into the heart of another unsensitized guinea-pig. 



TABLE XXIV 

 No. Fluid. Effect. 



1 .... Serum None 



2 .... Liver None 



3 .... Kidney Slight scratching 



4 .... Spleen None 



2. One milligram of egg-white incubated for thirty 

 minutes in 5 c.c. of the serum or organ extracts of a guinea- 

 pig killed three days after sensitization to egg-white is 

 without marked effect when injected into the heart of a 

 fresh guinea-pig. 



TABLE XXV 



No. Fluid. Effect. 



1 .... Serum Slight scratching 



2 .... Liver None 



3 .... Kidney None 



4 .... Spleen None 



5 .... Brain None 



3. With the conditions the same as in Table XXV, except 

 that the animal supplying the serum and organ extracts 

 had been sensitized to egg-white fourteen days before being 

 killed, the effects were marked as showed in Table XXVI. 



