PROTEIN SENSITIZATION OR ANAPHYLAXIS 277 



TABLE XXIX 



Time of 



No. Fluid. incubation. Effect. 



1 ... Serum 15 min. Dead in 30 minutes 



2 ... Serum 30 min. Dead in 6 minutes 



3 ... Liver 15 min. First and second stgaes 



4 ... Liver 30 min. Dead in 6 minutes 



5 ... Kidney 15 min. First and second stages 



6 ... Kidney 30 min. Dead in 5 minutes 



7 ... Spleen 15 min. First stage 



8 ... Spleen 30 min. First and second stages 



It seems from this that fifteen minutes is too short a 

 time for the full development of the poison. 



7. The ferment passes through hardened filter paper. 

 The serum and organ extracts from an animal killed twenty 

 days after sensitization to egg-white were filtered through 

 hardened paper. To each 5 c.c. of the filtrates 1 mg. of 

 egg-white protein was added, incubated for thirty minutes, 

 and then injections were made intracardiacly in fresh 

 guinea-pigs, with the results shown in Table XXX. 



TABLE XXX 



No. Fluid. Effect. 



1 .... Serum (3 c.c.) Death delayed 12 hours 



2 .... Liver First and second stages 



3 .... Kidney Dead in 8 minutes 



4 .... Spleen Dead in 6 minutes 



8. The ferment passes through a Berkefeld filter. The 

 serum and organ extracts of an animal killed twenty-two 

 days after sensitization to egg-white were filtered through 

 a Berkefeld V. To each 5 c.c. of these filtrates 1 mg. of 

 egg-white protein was added, incubated for thirty minutes, 

 and then intracardiac injections were made in fresh guinea- 

 pigs with results shown in Table XXXI. 



TABLE XXXI 

 No. Fluid. Effect. 



1 .... Serum Dead in 6 minutes 



2 .... Liver Dead in 9 minutes 



3 .... Kidney Dead in 4 minutes 



4 .... Spleen Dead in 6 minutes 



