PROTEIN SENSITIZATION OR ANAPHYLAXIS 281 



horse serum for thirty minutes. The serum and organ 

 extracts of an animal killed twenty-six days after sensiti- 

 zation to egg-white were incubated with horse serum 

 protein, 1 mg. to each 5 c.c. of fluid, for thirty minutes, 

 and then injected into the hearts of unsensitized animals, 

 with the results shown in Table XXXVI. 



TABLE XXXVI 



No. Fluid. Effect. 



1 .... Serum (2 c.c.) None 



2 .... Liver None 



3 . . . Kidney None 



4 .... Spleen None 



14. The serum and organ extracts of an animal sensitized 

 to horse serum do produce a poison when incubated with 

 horse serum. The serum and organ extracts of an animal 

 killed eleven days after sensitization with horse serum were 

 incubated for thirty minutes with 1 mg. of horse serum 

 protein to each 5 c.c. of fluid and then injected into the 

 hearts of fresh animals, with the results shown in Table 

 XXXVII. 



TABLE XXXVII 



No. Fluid. Effect. 



1 Serum Dead in 24 minutes 



2 .... Spleen Dead in 18 minutes 



3 .... Liver Dead in 27 minutes 



4 .... Kidney Dead in 60 minutes 



5 .... Brain Dead in 4 minutes 



15. The serum and organ extracts of an animal sensitized 

 to typhoid bacilli by the intensive method (described in 

 Zeitschrift /. Immunitatsforschnng, vol. ix, p. 458) when 

 incubated with living typhoid bacilli for thirty minutes 

 do produce a poison. This animal had been thus treated 

 twenty-seven days before. One-tenth of a loop from an 

 agar slant four days old was added to each 5 c.c. of fluid. 

 The results are shown in Table XXXVIII. 



