282 PROTEIN POISONS 



TABLE XXXVIII 



No. Fluid. Effect. 



1 . Serum Dead in 4 minutes 



Spleen Dead in 16 minutes 



Brain Dead in 4 minutes 



Liver Dead in 4 minutes 



Kidney Dead in 6 minutes 



16. A like experiment with the serum and organ extracts 

 of an animal treated twenty-eight days before with the 

 bacillus of cholera gave the results shown in Table 

 XXXIX. 



TABLE XXXIX 



No. Fluid. Effect. 



1 .... Serum Dead in 14 minutes 



2 .... Liver Dead in 6 minutes 



3 .... Brain Dead in 8 minutes 



4 .... Kidney Convulsions, recovery 



5 .... Spleen First and second stages 



17. Sensitization with egg-white in the guinea-pig does 

 not continue indefinitely. Vaughan and Wheeler found 

 that these animals, when the second injection was made 

 four hundred days or longer after the first, proved not to 

 be in a condition of sensitization, and that injections made 

 after this time resensitized. Apparently in cases of sensi- 

 tization with egg-white the serum first loses the specific 

 ferment. A guinea-pig killed thirty-four days after sensi- 

 tization to egg-white furnished a serum and organ extracts 

 which when incubated with egg-white and injected into the 

 hearts of fresh animals gave the results shown in Table XL. 



TABLE XL 



No. Fluid. Effect. 



1 .... Serum (3 c.c.) None 



2 .... Liver Dead in 7 minutes 



3 .... Kidney Dead in 18 minutes 



4 .... Brain Convulsions, recovery 



5 .... Spleen Convulsions, recovery 



