PROTEIN SENSITIZATION OR ANAPHYLAXIS 283 



18. When the amount of egg-white protein added to 

 5 c.c. of the fluid before incubation was varied, the results 

 as shown in the promptness and intensity of the effect of 

 the poison were found to vary. The serum and organ 

 extracts of two guinea-pigs, one killed twenty-eight days 

 and the other thirty days after sensitization to egg-white, 

 were divided into portions of 5 c.c., to which varying amounts 

 of egg-white were added, incubated for thirty minutes, and 

 injected into the hearts of fresh animals, with the results 

 recorded in Table XLI. 



TABLE XLI 



No. 



1 



2 



3 



4 



5 



6 



7 



8 



9 

 10 

 11 

 12 

 13 

 14 

 15 

 16 

 17 

 18 

 19 



Effect. 

 Slight 



Dead in 6 minutes 

 Dead in 4 minutes 

 Dead in 8 minutes 

 Convulsions, recovery 

 Dead in 2 hours 

 Dead in 5 minutes 

 Dead in 70 minutes 

 Dead in 2 hours 

 Convulsions, recovery 

 First and second stages 

 First and second stages 

 Dead in 80 minutes 

 Dead in 60 minutes 

 None 



Slight convulsions 

 Dead in 1 hour 

 Dead in 95 minutes 

 Dead in 65 minutes 



19. The protein poison prepared from egg-white by 

 cleavage with a 2 per cent, solution of sodium hydroxide, 

 and still by no means pure, kills guinea-pigs when injected 

 into the heart in doses of 0.5 mg. The minimum fatal dose 

 of the pure poison must be much less than this. The symp- 

 toms are the same as those induced by injections of the 

 serum and organ extracts from guinea-pigs sensitized to egg- 

 white after incubation with egg-white for thirty minutes. 



