PROTEIN SENSITIZATION OR ANAPHYLAXIS 299 



sensitized animal on reinjection. When the amount of 

 substrate employed was very small they obtained either 

 too little of the poison to affect the animal, or, what is more 

 probable, the digestion was so active that the poison itself 

 was destroyed. When whole blood corpuscles were used 

 the amount of poison obtained did increase with an increase 

 in the substrate, but the poison thus formed in greater 

 abundance was hemoglobin. At least this is our explana- 

 tion of their results. On the theory of Friedberger, his own 

 results are difficult, or, as we think, impossible of explana- 

 tion, while on our theory they explain themselves, and, 

 in fact, are exactly what might have been expected. 



Friedberger and Vallardi find that both the subjective 

 and objective symptoms of poisoning with anaphylatoxin 

 are identical with those of both active and passive anaphyl- 

 axis, and in this we quite agree with them. Biedl and 

 Kraus have held, and still hold, that anaphylatoxin cannot 

 be the true anaphylactic poison because, as they claim, 

 the condition of the lungs after death in guinea-pigs from 

 this poison is not the same. We agree with Friedberger, 

 who holds that the distention of the lungs after anaphyl- 

 actic death, first mentioned by Gay and Southard, and 

 more fully emphasized by Auer and Lewis, is found after 

 death from naturally poisonous sera, from poisonous anti- 

 sera, from peptone as shown by Biedl and Kraus, from the 

 poison of Vaughan and Wheeler, from the /3-i compound of 

 Barger and Dale, and possibly after poisoning from other 

 substances as well. Lung distention due to constriction 

 of the bronchioles is, in guinea-pigs at least, a constant 

 result of the protein poison, but should not be considered 

 as pathognomonic of this poison. Friedberger and Jeru- 

 salem 1 attempted to isolate and study the physical and 

 chemical properties of anaphylatoxin. It should be under- 

 stood that the poison as they had it is in guinea-pig serum. 

 They state their conclusions as follows: (1) The solution 

 can be evaporated to dryness (in vacuo) without loss of 



1 Zeitsch. f. Immunitatsforschung, 1910, vii, 748. 



