428 PROTEIN POISONS 



cancer cell and when the percentage of large mononuclear 

 leukocytes was at its highest point, the blood was collected 

 under sterile conditions, in 0.333 per cent, acetic acid. 

 This was next centrifugated until the leukocytes were 

 thrown down and the supernatant fluid decanted. The 

 leukocytes were then placed in a sterile mortar and mixed 

 with a sufficient quantity of sterile quartz sand to cover. 

 This was then ground up with vigor for fifteen minutes, 

 with the frequent addition of sterile normal salt solution, 

 until five times the volume of the leukocytes obtained had 

 been added. Next, this normal saline extract was separated 

 by passing through a Berkefeld filter and tests made for 

 the presence of the specific ferment by adding varying 

 amounts of the leukocyte extract to cancer-cell emulsion, 

 incubating for one hour and then injecting intravenously 

 into rabbits. Through repeated experiments it was ascer- 

 tained that 5 c.c. of 2 per cent, cancer-cell emulsion, plus 

 10 c.c. of leukocyte extract, which was obtained when the 

 percentage of large mononuclear leukocytes was 25 or 

 above, would, when injected intravenously into a rabbit 

 of from 500 to 1000 grams, kill within from one to five 

 minutes. This is well illustrated in the accompanying 

 table. 



TABLE XL VI. SHOWING RESULTS OF INJECTING INTRAVENOUSLY INTO 



RABBITS CANCER-CELL EMULSION PLUS LEUKOCYTE EXTRACT 



INCUBATED ONE HOUR. 



Weight, Rabbit leukocyte 



Animal. gm. extract. + Vaccine. Result. 



343 G. 646.5 10 c.c care. 100 5.0 c.c. 100 Died 1 min. 



167 G. 750.0 10 c.c. sarc. 51 res. 5.0 c.c. 100 Died 1 min. 



157 W. 1500.0 10 c.c. sarc. 100 5.0 c.c. 100 Very sick 



hr. ; rec. 



53 Bl. 1050.0 7 c.c. sarc. 51 res. 5.0 c.c. 100 Died 5 min. 



96 G. 1317.0 7 c.c. sarc. 51 res. 2.5 c.c. 103 Died 4J hr. 



251 W. 645.0 10 c.c. sarc. 51 res. Not sick. 



251 Br. 842.0 15 c.c. care. 100 Not sick. 



167 W. 700.0 10 c.c. normal sal. 5.0 c.c. 100 Not sick. 



The entire mass of leukocytes, when removed from the 

 outside of the Berkefeld filter and mixed with 5 c.c. of 



