SPECIFIC FERMENTS OF THE CANCER CELL 429 



cancer-cell emulsion and incubated one hour, failed to have 

 any effect on a rabbit when injected intravenously, thus 

 showing that the specific ferment was soluble. 



Again, 10 c.c. of leukocyte extract, prepared in the same 

 manner from a normal rabbit which had not been sensitized, 

 added to 5 c.c. of cancer-cell emulsion and incubated one 

 hour, produced no effect when injected intravenously into 

 a normal rabbit. This would show that the sensitized 

 animal possesses some specific chemical substance which 

 reacts with cancer tissue and which the normal rabbit does 

 not possess. That this substance is specific for malignant 

 cells is further shown by the fact that 10 c.c. of leukocyte 

 extract from a sensitized animal, plus 5 c.c. of a 2 per 

 cent, normal skin vaccine, incubated one hour, produces 

 no effect when injected intravenously into a rabbit of 

 450 grams. 



The same table shows also that sarcoma residue or 

 vaccine sensitizes to carcinoma as well as sarcoma, and 

 vice versa, so that the probable conclusion is that the chemical 

 change within the cell is the same for both sarcoma and 

 carcinoma. 



Organ extracts from kidney, liver, brain, spleen, and 

 heart, made by grinding these organs in normal saline on 

 successive days, after sensitization to cancer protein, have 

 no effect when mixed with cancer-cell emulsion, incubated 

 one hour; and injected intravenously into normal rabbits. 



When as small an amount as 1 c.c. or more of leukocyte 

 extract is injected directly into the tumor of a cancer 

 patient it may cause sudden and severe symptoms. In four 

 cases, when this procedure was adopted, the patient has 

 complained within from one to five minutes of difficulty 

 of respiration. Next, he would lose consciousness, which 

 would be accompanied by rather violent muscular twitchings 

 and lowered pulse-rate. This stage would last from five 

 to ten minutes, and would be followed by a period of rest, 

 from fifteen minutes to one hour in duration, which in turn 

 would be followed by a violent chill, and temperature 

 ranging from 103 to 106 F. This would last from one to 



