432 PROTEIN POISONS 



hot water. It was then washed with alcohol and ether, 

 and dried to a constant weight at 110. 



Weight of globulin and filter ....'. 1.5864 grams 

 Weight of filter 1.5766 grams 



Weight of globulin . 0098 gram 



25 c.c. of the same leukocyte extract after Berkefeld 

 filtration was treated in the same manner. 



Weight of globulin and filter 1.4913 grams 



Weight of filter 1.4766 grams 



Weight of globulin 0.0147 gram 



By subtracting these globulin weights of before and 

 after filtration from the above-given albumen plus globulin 

 weights we arrive at the albumin weights: 



Albumin and globulin before filtration . . 0.05508 gram 

 Globulin before filtration 0.00980 gram 



Albumin before filtration 0.04528 gram 



Albumin and globulin after nitration . . 0.01875 gram 

 Globulin after filtration 0.01470 gram 



Albumin after filtration . 00405 gram 



From the above it can be seen that we have removed a 

 large percentage of the albumin through the passing of the 

 extract through the Berkefeld filter. This is represented 

 by the difference between 0.04528 gram and 0.00405 gram, 

 which is 0.0412 gram. 



The difference in the globulin weight gives an apparent 

 increase of 5 mg. in the after-globulins, an amount so small 

 that it can be neglected when compared with the albumin 

 difference. 



From this it can be seen that the sensitizing portion of 

 the extract is in all probability contained within the albumin, 

 and that the specific enzyme is not of albuminous nature. 



