SMALLPOX, SYPHILIS, CANCER, ETC. 197 



reported that the growth was a typical squamous-celled 

 cancer. 



After removal of the gland, which was of the size of a 

 small walnut, an incision was made into it, revealing a 

 central cavity lined by a layer of yellowish material about 

 2 mm. in depth, easily separable from the firmer tissue 

 beneath. Sections were made by Dr. Wyatt Wingrave, 

 pathologist, who stained them with pyronin and methyl 

 green. 1 



The section I have is beautifully stained and well fixed. 

 It shows that a layer of healthy parotid was cut away with 

 the tumour, and that the latter is a typical squamous-celled 

 cancer. There are in the younger epithelial cells what 

 appear to be unusually prominent nucleoli. On close 

 inspection some of the latter are seen to have escaped from 

 the nuclei and then to have expanded suddenly, i.e. before 

 getting clear of the host-nucleus, and to have become them- 

 selves nucleated, and with their nuclei to be dividing by 

 direct cell-division. 



The process in this squamous-celled cancer was the same 

 as that of the free parasites of cancer of the uterus described 

 in Part III. 



Spirochaetes in cancer. Spiral forms of parasites con- 

 sisting almost entirely of chromatin I described in Part II 



1 The following are the details of the method : 3 parts aqueous solution of pyronin ; 

 1 part saturated solution of methyl green. Stain five minutes or longer, transfer to 

 fresh solution of resorcinol (3 grains to watch-glass) for one minute, then to solution 

 of resorcinol in absolute alcohol (0*6 grain to watch-glass) till sufficiently decolorised ; 

 then three changes of absolute alcohol, and two of xylene. Mount in dammar-xylene. 



