ABBREVIATIONS, &c. 



M.G. and S. stands for my " Morbid Growths and Sporozoa " ; condensed 

 from " Cancer, Sarcoma, and other Morbid Growths Considered in 

 Relation to the Sporozoa," published in 1893. 



Parts I, II, III, and IV, stand for the parts of my " Protozoa and Disease," 

 published in 1903, 1908, 1912, and 1915, respectively. 



H. and E. means haematoxylin and eosin ; i.e. acid haematoxylin made 

 according to Ehrlich's formula, and Gruebler's water-soluble 

 eosin ; the stains being used in the ordinary way. 



E.-B.-H. means Ehrlich-Biondi-Heidenhain stain. 



Ehrlich's acid haematoxylin : haematoxylin 2 gm. dissolved in alcohol 

 abs. 100 c.c. ; add water dist. 100 c.c. ; and acetic acid, glac. 10 c.c. ; and 

 alum, to saturation. 



The stain must " ripen " by being kept in the light and having the 

 stopper of the bottle removed to admit air from time to time. When ripe 

 it has a dark red colour, the haematoxylin having been oxidised to haema- 

 tein. The mixture can be kept for years without deterioration. It answers 

 well for staining in bulk. For staining sections it is diluted with distilled 

 water, sections become a brown colour, the blue colour being developed in 

 tap-water. 



For counterstain, water-soluble eosin (Griibler's) kept in alcoholic 

 solution is mixed with water, a few drops to a watch-glass. 



Ehrlich-Biondi-Haidenhain triple stain. Saturated aqueous solutions 

 of orange (100 c.c.), acid fuchsin (20 c.c.), methyl green (50 c.c.) are mixed 

 in that order with constant stirring. Dehydrate rapidly with alcohol, 

 clear in xylol and mount in xylol-balsam. This stain is brilliant but fades 

 so rapidly that I do not now have it used when a permanent preparation 



is required. 



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