352 M. H. Karsten on Fatty 



twenty to twenty-four hours. By observations repeated every 

 two liours during this time we may very easily follow the whole 

 course of development step by step, and ascertain by effecting 

 a contraction of the secondary cells by the employment of 

 diosmotic agents, or by sudden considerable alteration of tem- 

 perature, &c., that in fact the production and development of 

 these hysterophymata takes place within these cells. In from 

 eighteen to twenty hours the first indications of bluing by 

 iodine usually occur ; we find individual joints of the Vihriones 

 somewhat inflated, the terminal joints most frequently, and 

 these also separated and coloured blue by iodine in the midst 

 of the great mass of yellow Dicoccij Vihriones, &c. which were 

 developed somewhat later and more slowly. 



The development of the hysterophymata takes place free in 

 the nutritive fluid, Vv^hich always contains the contents which 

 have flowed out of the torn or cut cells, and also in the cut 

 cells themselves, much more rapidly than within the closed 

 cells ; and we may see several or all the joints of a 

 Vibrio simultaneously develop daughter cells, inflated in 

 various forms, and become coloured blue by iodine, while the 

 germs enclosed in the cells have scarcely commenced their 

 evolution. But all the cells of the tissue of a beetroot &c. do 

 not behave in the same manner ; nor do all simultaneously 

 develop the same forms in their interior, but earlier or later 

 according to the more or less albuminous nature of the con- 

 tents. Hence the elongated cambium-cells in the neighbourhood 

 of the vascular bundles are earlier than the cells of the paren- 

 chyma ; whilst of the latter again some are earlier than the 

 others, according to the constitution of their continually 

 changing contents, which is dependent upon their age and 

 their chemical stage of development. 



The cells nearest the surface are also naturally much earlier 

 and more intensely penetrated by the nutritive fluid and ex- 

 cited to the new formation above described than those situated 

 more in the centre of the organ, which frequently develop 

 none of the large vibrioniforai cell-structures, becoming blue 

 with iodine when some time has elapsed since the commence- 

 ment of the process. In these cells Vihriones certainly are 

 developed, but only albuminous ones, which are colom-ed yellow 

 by iodine and reddish by Millon's salt. 



It would appear that for the evolution of the amyloid hyste- 

 rophymata an organic compound soluble in water is necessary, 

 which diffuses itself outwards from the cells situated in the 

 interior of the tissue, where the latter is permeated by an in- 

 sufficiently concentrated solution of nutritive material, — and 

 that, on the other hand, the salts of the nutritive material are 



