58 DEPARTMENT OF AGRICULTURE. 



2. By the inothod that I emplbyed, one hundred and eight beasts 

 were preserved from phMiro-pneumoiiia, wliile of fifty beasts placed in 

 the same stables and not inoculated, seventeen became diseased, and 

 the disease is now banished from these stables, which had never been 

 free from it since 1830. 



3. The inoculation of the disease itself, i^erformed in the manner that 

 I have described, whether it may have occasioned apjiarent morbid 

 manifestations or not, was the measure that preserved the animals from 

 plem'O-pneumonia. 



4. The blood and the serous and frothy liquid squeezed from the lungs 

 of a diseased animal in the first stage of pleiu'O-pneumonia is the most 

 suitable matter for inoculation. 



5. The inocidation of the virus takes from ten days to a month before 

 it manifests itself by sensible symptoms. 



6. The matter emj^loyed for the inoculation has, in general, no effect 

 upon an animal preNaously inoculated or having had the disease. 



7. The inoculated animal braves the epizootic influences with impu- 

 nity, and fattens better and more rapidly than those in the same atmo- 

 sphere with it that have not been inoculated. 



8. The inoculation should be performed with prudence and circum- 

 spection upon lean animals in preference, and towards the tenth day 

 after the operation a saline purge may be given, and repeated if neces- 

 sary. 



9. By inoculating pleuropneumonia a new disease is produced; the 

 affection of the lungs, with all its peculiar characters, is localized in 

 some sort on the exterior. 



10. The virus obtained from oxen affected with pneumonia is of a na- 

 ture entirely specific ; it does not always act as a virus ; the bovine race 

 alone is affected hy its inoculation, while no other animals of different 

 races, inoculated in the same manner, and with the same liquid, experi- 

 ence any ill effects. 



Dr. Willems accomplished much in his earlier experiments, as will be 

 seen by comparing the knowledge of the present day with the results of 

 his original investigations. One cause contributed to strengthen the 

 hands of his adversaries, and this was attempting to prove that specific 

 and characteristic elements distinguished the virus of pleuropneumonia. 



Dr. AVillcms says : 



I have exiiiniiK (1 various iiathological specimens Avitli the object of studying and 

 clncidating thi' (lucstion of inoculation. My investigations have been principally 

 directed to diseased lungs, and to a kind of tubercle hitlu-rto overlooked, but whieb I 

 have, nevertheless, constantly met with upon openiug the dead bodies ol" animals that 

 died from i)lenro-pneumonia. These tubercles, scattered throughout the iutestines, but 

 principally in the lesser one, are of a size varying from the head of a pin to that of a 

 large pea, of a yellowish or greenish color; they are seated in the submucous cellular 

 tissue, and partly in tlie thickness of the nuuMius nieml»rauo of the intestine. Tlu'y do 

 not ;i|iiie:ir to linve any relation with the glands of Peyer or of" Hrunner. Are they 

 hypertrophied follicles ? Nothing appears to prove it ; no openiug is perceived in them . 



