SG DEPARTMENT OF AGRICULTURE. 



llow many recovor their health after having been snbjected to this test, 

 and nnder what conditions? 



Fifth. ]3o the animals snbjected to this jnoof of inocnlation with jml- 

 monary li(|uid acciniic the i)0\ver of resisting the contagion of i>leiiro- 

 pnenmonia? 



The experiments made to solve the qnesticni whether plenro-pnenmonia 

 was contagious by the inocnlation of the blood, saliva, nasal mucus, &c., 

 having been performed only on six animals, the commission has not 

 deemed them suflticient in number to form the basis of any conclusion. 

 Nevertheless, it was thought right to mention that the two cows inocu- 

 lated with the misal discharge, and subjected to the proof of contagion 

 by cohabitation, have not been affected with pleuro-pneumonia. 



Experiments by inoculating the liquid from the lungs of sick cattle 

 have been performed on fifty-four healthy animals, and under conditions 

 which indicated that these animals had never previously contracted the 

 disease. Of these fifty-four subjects inoculated none have shown symp- 

 toms of i)leuro-pneumouia as the result of inoculation. On twenty-three 

 the effects of inoculation have only been indicated by a slight local and 

 well-circumscribed inflammation. On twenty-one the inflammation has 

 been very severe, very extensive, and complicated by gangrenous phe- 

 nomena which have led to the death of six subjects. Therefore the num- 

 ber of animals in which inoculation has been benignant has amounte<l to 

 Gl.ll per cent.; the proportion of those having gangrene after the oper- 

 ation, Avhich resulted in the loss of a portion of the tail, was 27.77 per 

 cent.; lastly, the deaths attained 11.11 per cent. Thus 88.88 per cent, 

 of the inoculated animals recovered, and 11.11 per cent. died. 



Of the forty-eight subjects which came out of the inoculatioTi safe and 

 healthy two died of accidents not induced by the operation, and thirty- 

 four were exi)osed for a period of five or six months to the direct influ- 

 ence of contagion by cohabitation with twenty-four subjects that had not 

 been inoculated, and which had to serve as a means of comparison. 



Twelve iiu)culated animals which had been placed in separate stables 

 to serve for ultefior exi)eriments were not exposed to the direct contact 

 of such cattle, but were looked alter by the same person who had charge 

 of the sick animals. 



Only one of the forty-six animals inoculated, viz., about two per cent., 

 became atfected with pleuro-pneumonia, whereas of the twenty-four non- 

 inoculated animals fourteen, or fifty-eight per cent., suffered. 



From these experiments the commission concludes: 



1. The inoculation of the liquid extracted from the lungs of an animal 

 affected witli i)leui<)-])ueumonia does not transmit to healthy animals of 

 the same s[>ecies the same disease — at all events, so far as its seat is con- 

 cerned. 



2. The a])preciable plienomena which follow the inoculation are those 

 of a local inllammatiou, which is circumscribed and slight, on a certain 

 number of t lie auimals inoculated; extensive and diffuse, with general 

 reaction proi>ortioned to the local disease, and complicated by gangrenous 



