180 DEPARTMENT OF AGRICULTURE. 



of ten (lays cnreAil exaiuiuatioii failed to discover any organic; forms in 

 either of the fhisks. 



Experiment 5, Febrnary 25, 18C9.— One of the vacunm tubes of 

 Wood from tlie above-mentioned cow, and a tube of healthy blood wliich 

 had been put up at tlie same time, were opened and carefully examined. 

 The blood in each was coagulated, free from ofi'ensive odor, and under 

 the microscope presented no nnusual appearance. The contents of each 

 tube were placed in a one-ounce vial with a slip of purified blotting pa- 

 per, the vials sealed and kept at a temperature of 70° Fahrenheit. Ten 

 days later bacteria and vibriones were present in each, but no trace of 

 mycelium or of fungus fructification. 



On the 2Gth of February, 18G!), a cow in the last stages of pleuro-pneu- 

 monia was killed near Washington, and vacuum tubes filled from the 

 jngular vein. Tubes were also filled with the serum contained in bulla? 

 fornuHl by the false membrane lining the bronchial tubes. 



About four inches of each jugular vein were removed, ligatures having 

 been first applied. Eighteen hours afterwards the blood in the veins 

 from which the tubes had been filled was carefully examined with a 

 power of seven hundred and fifty diameters. It was coagulated, and 

 the serum contained some molecules, single or in chains of two or three, 

 which were motionless, (Fig. 1, PI. 1.) Blood from one of the f acuum 

 tubes contained no such bodies. The lung serum contained molecules 

 like those in the vein. 



Experiment 6, February 26, 18G9. — In a culture apparatus were 

 placed three watch-glasses and two growing slides, arranged as follows: 

 The growing slides and watch-glass No. 1 contained boiled potato and 

 diseased blood ; watch-glass No. 2 contained boiled potato and healthy 

 blood; watch-glass Xo.3 contained boiled potato and lung fluid. Twenty- 

 four hours later, in the growing slides the red corpuscles had nearly dis- 

 appeared; bacteria and monads, single or in short chains, were -seen': a 

 few moving, but the greater i^art at rest. Seven days later there was 

 no change; motionless bacteria and monads were present in all the 

 glasses, but no trac^e of mycelium or spores. 



Experiment 7, February 20, 1800. — Seven watch-glasses and five 

 gTowing slides were arranged as follows : AVatch-glass No. 1 contained 

 j)Otato l)oiled in distilled water; watch-glass No. 2 contained lemon boil- 

 ed in distilled water; watch-glass No. o contained lemon boiled with dis- 

 ease<l blood; watch-glass No. 4 contained diseased blood alone; watch- 

 glass No. 5 contained healthy bhxxl alone; watch-glass No. contained 

 boiled potato with diseased blood; watch-glass No. 7 contained boiled 

 potato with healthy blood; growing slide A contained boiled lemon with 

 diseased blood; growing slide B contained boiled lemon with liealthy 

 blood; growing slide ('/ containecl boiled potato with diseased blood; 

 growing slide 1) contained lK)ile(l ])()tato with healtliy blood ; growing 

 slide Fj contained boiU'd i)()tato alone. Tiiese were i)laced in four sets 

 of culture apparatus, and kept at a temperature of 78^^ Fahrenheit. lu 



