4:8 BULLETIN 1059, U. S. DEPARTMENT OF AGRICULTURE. 



seedlings later being thinned to uniform density. Unless it is desired 

 to determine the effect of light on germination, this process should 

 be concluded for all pans, under uniform conditions, before they are 

 placed in the solarium. The main operations may be started just 

 as soon as the seedlings are established. Otherwise great care must 

 be used to develop the seedlings similarly in all pans. 



Having reached the proper stage, place 10 pans in each of the three 

 compartments; say, in two north-and-south rows. The three com- 

 partments are to be maintained at different temperatures; say. at 

 mean temperatures of 50°, 60°, and 70° F., with more or less diurnal 

 oscillation in each. In each compartment one row of pans is to be 

 given sufficient water to maintain its soil moisture at, say. twice the 

 wilting coefficient, while the other row will be maintained at four 

 times the wilting coefficient. The condition of the pans may at any 

 time be determined by weighing and the water supply regulated 

 accordingly. In any row of five pans five different light intensities 

 may be maintained. One pan in each row should doubtless be 

 allowed full sunlight, another should be cut 20 per cent, a third 40 per 

 cent, etc. The amounts may be governed by previously gained 

 empirical knowledge of the requirements, so that the full range of 

 light values will not have to be covered in any case. 'Die shading 

 of each pan separately may be arranged by using covers of punched 

 screen, in which the areas of the openings correspond to the proportion 

 or full light which it is desired to admit. It should be borne in 

 mind at the outset that the glass of the solarium considerably reduces 

 the light intensity, particularly in the infra-red rays. The quality 

 of this light should be compared with that of direct sunlight, and 

 means should also be devised for measuring the light intensity under 

 each screen. 



The proper heating of the various compartments will prove the 

 most serious obstacle in most cases. This, of course, will have to he 

 accomplished by artificial means and should be done by introducing 

 warm air into the compartments from an outside source in such a 

 manner as to maintain the desired air temperatures without directly 

 heating the pans. The air thermometer and thermostat should be 

 suspended at a mean elevation and protected from insolation. 



The positions of pans in each compartment should be frequently 

 changed so that none will profit more than others by Localized heat 

 and light optima, which are certain to exist. 



The final effect of light and also the effect of other factors with 

 light is to be determined by the accretion of dry matter in the seed- 

 lings of each pan and species. In order that tins may be expressed 

 in net quantities for the time of treatment, some of the seedlings 

 weeded out at the beginning of the test should be dried and weighed. 



It is evident that this general plan might be followed in tests to 

 show the effect of different kinds of light on growth, using mono- 

 chromatic screens as covers for the pans instead of the punched me- 

 tallic screens, or supplying different compartments of a dark chamber 

 with various kinds of artificial light. 



4. The fourth method of determining tolerance or light require- 

 ments is similar to that just described, but depends on the measure- 

 ment of light intensities as they are encountered in the field, and the 



