REACTION OF CELLS 



65 



in a given time, e.g. 10 minutes. If, for instance, we 

 find that the fourfold quantity is necessary for 

 reaching the same haemolytic effect at 29 C. as at 

 39 C., we may say that according to the ^/-rule 

 the same quantity of ammonia would occupy a time 

 four times as long to produce the same effect at 29 

 C. as at 39 C. 



Such determinations have been carried out on a 

 very large scale with different haemolytic agents by 

 MADSEN and his co-workers WALBUM and NOGUCHI. 

 As an instance, I give a series for ammonia with a 

 time of action of 10 minutes. / is the temperature, q 

 the necessary quantity in cc. of a 05 normal NH 3 

 solution. The total quantity was 8 cc. containing i 

 per cent of red blood -corpuscles from a horse. 

 ^ obs> is the observed, ^ calc-) a calculated quantity 

 evaluated by means of the general formula for the 

 influence of temperature on the velocity of reactions. 

 The degree of haemolysis was 1 7 per cent. 



HAEMOLYSIS BY MEANS OF AMMONIA AT DIFFERENT 

 TEMPERATURES 



The value of ^ used for the calculation, which 

 agrees very well with the observation, is 26,760. 



F 



