130 CHEMICAL EQUILIBRIA 



of the observed degree of haemolysis in accordance 

 with experiments of MANWARING. The quantity of 

 haemolysin necessary for complete lysis is termed 

 100. 



EQUILIBRIUM BETWEEN AMBOCEPTOR FROM GOAT, COMPLEMENT 

 AND HAEMOLYSIN FOR Ox ERYTHROCYTES 



The tabulated quantity is the concentration x of haemolysin. 



In brackets are written values calculated from the 

 formula 



(50 -x) (206 -x) = gox. 



The agreement between the observed and the 

 calculated quantities is quite sufficient, considering 

 the rather large errors of observation. It is quite 

 clear that the quantity of haemolysin increases both 

 with the quantity of amboceptor and with the 

 quantity of complement used. But even with the 

 greatest quantity of complement (b = 60) we do not 

 reach complete haemolysis (x= 100) if there is not 

 a sufficient quantity of amboceptor (a = 20) present. 

 In this case x according to the formula cannot ex- 



