132 CHEMICAL EQUILIBRIA 



and amboceptor from rabbit with guinea-pig serum 

 corresponded to the formula 



In the first of these two we find amboceptor from 

 goat and guinea-pig serum just as in the example 

 given in detail above (p. 1 29). The only difference is 

 that the red blood-corpuscles were taken from sheep 

 in the one case and from ox in the other case. The 

 sheep is much more nearly related to the goat 

 than the ox is. This relationship finds its ex- 

 pression in the dissociation constant 1900 for the 

 combination sheep-goat as compared with the dis- 

 sociation constant 90 for the combination ox-goat. 

 The higher the dissociation constant the less is the 

 tendency to form the compound haemolysin. The 

 more the animal in which the erythrocytes are in- 

 jected differs from that which has supplied the 

 erythrocytes, the easier is the formation of the 

 haemolysin. The attempt to produce a haemolysin 

 by injection of red blood-corpuscles of one animal 

 into the blood of another animal of the same species, 

 therefore, seldom meets with success. Still there 

 are some reports that so-called isolysins have been 

 obtained with such a treatment (EHRLICH and MOR- 

 GENROTH, 1900). But in this last case, with goat 

 serum, it was necessary to use thirty times as much 

 amboceptor for reaching complete haemolysis of 

 goat corpuscles as with goat serum against ox 

 corpuscles, which makes an extremely high dis- 

 sociation constant probable. 



