ESTIMATION OF HEMOGLOBIN 



11 



cover glass of Zeiss with the central excavation. After the count- 

 ing chamber is filled, it is let stand 10-15 minutes to let the plate- 

 lets settle. The red corpuscles are decolorized and appear as 

 " shadows." The nuclei of leucocytes are stained dark blue. The 

 platelets appear as sharply outlined round or oval or elongated 

 lilac colored bodies. 



After the blood and diluting fluid are mixed precipitate does 

 not form. The count may be made after some hours if necessary. 



The cresyl blue solution is stable but should be kept on ice to 

 prevent the growth of mould. The cyanid solution should be 

 made up at least every ten days. 



This apparatus consists of 



ESTIMATION OF HEMOGLOBIN 



There are several instruments for obtaining the amount of 

 hemoglobin, the more important of which are Tallqvist's, Gower's, 

 Dare's and Fleischl-Miescher's. 



Tallqvist's hemoglobin scale, 

 fifty leaflets of absorbent paper 

 bound in a booklet with a scale 

 of ten standard tints correspond- 

 ing to the color of blood stains 

 having a hemoglobin value of 10, 

 20, 30, 40 to 100. In making the 

 test a small piece of the absorb- 

 ent paper is touched to a drop of 

 blood which is allowed to soak in 

 gradually. As soon as the blood 

 has lost its humid gloss and be- 

 fore drying has taken place the 

 stain is placed against a white 

 background and compared with 

 the tints of the standard scale. 

 The tints should be compared by 

 daylight. This is one of the most 

 convenient of the hemoglobinom- 

 eters. The. booklet is of a size FlG - 6 - Tallqvist's hemoglobin scale. 



convenient to be carried in the pocket, requires only a few seconds 

 to make a test and has no pipettes or other parts to be cleaned. 



