TEST FOR FAT 23 



or five minutes. The glycogen appears as reddish brown granules 

 in the cell bodies of the polymorphonuclear leucocytes, rarely of 

 basophiles and myelocytes or as a diffuse reddish brown coloration 

 of the cell bodies and as small or larger masses similarly tinted 

 outside the corpuscles. A small amount of extra cellular glycogen 

 may be found in normal blood. 



TEST FOR FAT 



The presence of fat may be proven by fixing a smear for 24 

 hours in one per cent, osmic acid. The specimen may be counter- 

 stained with eosin. A control smear should be fixed 24 hours in 

 alcohol and ether, then in one per cent, osmic acid for 24 hours. 

 A control specimen in which the fat is dissolved by ether is needed, 

 since osmic acid will blacken other substances than fat. Fat may 

 be detected with greater certainty by staining with Scharlach R 

 or Sudan III. Films spread on slides are fixed at once, before 

 drying takes place, in formaldehyde vapor for five or ten minutes 

 and are then stained in a saturated alcoholic solution of Scharlach 

 R or Sudan III for 15 or 20 minutes, preferably in a tightly stop- 

 pered bottle. Wash in water, mount in glycerin and examine with 

 two mm. oil immersion objective. 



THE TOTAL VOLUME OF BLOOD AND ITS OXYGEN CAPACITY 



The importance of knowing the total quantity and oxygen 

 capacity of the blood is evident. Haldane and Smith have de- 

 vised a method for determining these. A small and carefully 

 measured quantity of carbon monoxide is administered and then 

 the percentage to which the hemoglobin has become saturated is 

 determined by the carmine method. This gives data to deter- 

 mine the total volume of CO (or oxygen) capable of being taken 

 up by the blood. At the same time the volume of CO (or of oxy- 

 gen) capable of being taken up by 100 grams of blood is deter- 

 mined by comparing its color with that of an equal volume of ox 

 blood whose oxygen capacity has been determined. This will 

 give data to determine the total quantity of blood. Haldane and 

 Smith found that the total amount of blood in man is about 4.9% 

 of the body weight and varied in 14 healthy persons between 3.34 

 and 6.27%. The total oxygen capacity they found to be 0.85% 



