RINDERPEST 149 



Nicholle and Adil-Bey have shown that the virus is able to pass 

 through a Berkefeld filter. The changes in the leucocytes in the 

 course of the disease have been studied by Refik-Bey. He found 

 that in cases of fatal infection there is an initial increase in the 

 number of leucocytes followed by a decrease, constantly present; 

 then there is a secondary increase, constant!)' present. The initial 

 increase takes place the second or third day after inoculation; the 

 number may reach 18,300 per cmm. The mononuclears, including 

 the lymphocytes, increase in about half the cases, maximum ob- 

 served 12,300 per cmm. Refik-Bey states that the normal number 

 of mononuclears in cattle is from 4,500 to 6,500 per cmm., 57-84%; 

 the normal number of polynu clears 1,500 to 3,500 per cmm. Gen- 

 erally the polynuclears take part in the initial increase, maximum 

 observed 8,000. Sometimes the eosinophiles are increased, maxi- 

 mum observed 3,500. 



The number of leucocytes commences to decrease the fourth 

 day (sometimes the third), the minimum being ordinarily reached 

 the fifth day (sometimes the fourth, exceptionally the sixth or 

 seventh). The lowest count obtained was 2,000. The minimum 

 is generally observed the day of the rise of temperature, rareby the 

 day before and sometimes the second or third day of fever. In 

 this stage all the varieties are diminished. The minimum number 

 of mononuclears observed was 1,000 per cmm., of polymorphs 200 

 per cmm., of eosinophiles 200 per cmm. The eosinophiles then 

 disappear and do not reappear. 



The secondary increase begins generally the eighth day, some- 

 times the seventh and exceptionally the ninth. Usually the tem- 

 perature falls at this time; rarely it has fallen the day before or 

 falls the day following the increase. The leucocytes in the cases 

 observed have increased, exceeding the normal; in one case there 

 were 45,000 per cmm. Toward the time of death the leucocytes 

 commence to decrease. The mononuclears increase, in half the 

 cases observed not reaching the normal; in the other half reaching 

 or exceeding the normal, maximum 27,000 per cmm. The poly- 

 morphs increase, sometimes simpty returning to the normal 

 number and sometimes exceeding it. The polynucleosis is more 

 marked when life is prolonged, maximum observed 18,000 per 

 cmm. 



