Miscellaneous. ATT 
muscles presented a profound alteration, which explained the en- 
feebling of the animal’s movements. 
To study the Bacteria more completely I collected a drop of blood 
from the Yalitrus and added to it a drop of gentian-violet. Thus 
treated the Bacterian was brightly coloured. It presents the form 
of a Diplobacterivm measuring about 2 »; each of the geminate 
joints is less than 1 p. ‘There are also chaplets of three or four 
joints, rarely more, and here and there a few isolated bacilli, a little 
longer (3-5 1). 
The phosphorescent disease being manifestly of an infectious 
nature, I tried inoculations upon Talitrt and Orchestie (0. littorea, 
Mont.). For this purpose I cut off two more legs of the luminous 
Talitrus. Each of these was torn up separately in blood of Talitrus 
and of Orchestia; then with a sterilized needle I pricked the Talitri 
and the Orchestie on the sides of the body, taking care not to wound 
the liver or touch the dorsal vessel, in order to avoid a too abundant 
hemorrhage. I then applied a drop of virus to the wounded places, 
and the inoculated animals were enclosed in glasses furnished with 
a thin layer of sand, and covered over and placed in the cellar of the 
laboratory at the temperature of 59°-64° F. 
The result exceeded my expectations. Of the Talitri inoculated 
on the 6th September six began to shine on the 8th and appeared 
on the evening of the 9th as brilliant as the first luminous Yalitrus. 
Out of a dozen Orchestie inoculated the same day three became 
phosphorescent on the 9th and were resplendent on the 10th. I 
have since continued the inoculations, operating about every two 
days ; and I possess at present Yalitri of the sixth luminous genera- 
tion and Orchestiw of the fourth generation. The action of the 
microbe does not seem to diminish at all, and in the evening the 
cellar of the laboratory presents a fairy aspect, which is the admira- 
tion of the bathers staying at Wimereux. 
The Bacteria is not modified by passing into the Orchestia ; Talitri 
inoculated with virus taken from Orchestie of the third generation 
behaved as if they had been infected by the blood of other Valitri. 
The disease follows a very regular course. At first one sees only 
a luminous point at the place of the puncture. After the lapse of 
from forty-eight to sixty hours the whole animal is phosphorescent, 
but with a white light which has little external diffusion. At this 
time the Talitrus still shows great activity. After the third or 
fourth day the phosphorescence becomes brilliant and of a fine 
greenish tint and the animal throws out a bright light around it. 
It may be perceived at a distance of 10 metres, and two Yalitri 
suffice to enable one to see the time by a watch as in full daylight. 
At this phase of the malady the Talitrus progresses more slowly ; it 
can still issue from its burrow, which it illuminates, and return 
there if disturbed. The period of this state may last from three to 
six days; then comes a period of immobility, during which the 
phosphorescence retains all its brilliancy. Lastly, in three or four 
more days the animal dies; the body remains phosphorescent for 
some hours and then acquires a very characteristic brown tint. 
Frequently the point of inoculation is surrounded by a small blackish 
circle. Lowering of the temperature seems to prolong the life of 
the animal; Yalitri inoculated on the 9th September and kept at a 
temperature of 50°-57° F. were still living on the 22nd September. 
