478 Miscellaneous. 
In the Orchestie the inoculations do not succeed so easily because 
the operation is more delicate; but the animal longer retains its 
muscular power: an Orchestia inoculated on the 12th still jumped 
on the 19th, although it was in full phosphorescence. The TYalitri 
and Orchestiw in which the inoculation does not succeed remain in 
perfect health long after their congeners are dead; the puncture, 
when well made, therefore is not serious in itself. 
I have inoculated examples of Hyale Nilssoni, Rathke, with perfect 
success ; in these little Amphipoda phosphorescence is produced in 
forty-eight hours. Specimens of Ligia oceanica, Linn., though more 
resistant, also gave a favourable result. Of six Ligice unsuccessfully 
inoculated on the 10th and reinoculated on the 16th only one was 
infected ; but after the 20th it presented an admirable spectacle. 
I have also succeeded in inoculating crabs (Carcinus menas, 
Linn., and Platyonychus latipes, Penn.). In these animals, how- 
ever, the morbid phenomena are much more complex, and I will 
notice them in a subsequent communication. At the same time I 
will describe my experiments in the culture of the Bacteria in arti- 
ficial media.—Comptes Rendus, September 23, 1889, p. 503. 
On the Parasitic Castration of the Typhlocybe by a Hymenopterous 
Larva (Aphelopus melaleucus, Dalim.) and by a Dipterous Larva 
(Atelenevra spuria, Mezg.). By M. A. Grarp. 
The Hymenopterous and Dipterous larve parasitic upon Typhlocyba 
noticed by the author in a former communication * belong, the 
former to Aphelopus melaleucus, Dalm., the latter to Atelenevra 
spuria, Meig. (A. velutina, Macq., Chalarus spurius, Schin.). 
These insects, like their hosts the Zyphlocybe, have two genera- 
tionsin the year: one, proceeding from pupze formed during the second 
fortnight in June, comes out at the beginning of July; the other 
infests the second generation of Vyphlocyba, enters the pupa state 
towards the end of September or in October, and probably passes 
the winter in that state, producing the perfect insect in the following 
spring. 
Combining these observations with those of Perris (on the parasi- 
tism of Dryinus pedestris, Dalm., upon Athysanus maritimus, Perris) 
and of J. Mik (on the parasitism of Gonatopus pilosus, Thoms., upon 
Deltocephalus xanihoneurus, Fieb.) it seems probable that the Proc- 
totrupians of the family Dryinide are generally parasitic upon 
Homoptera of the family Jassidee, 
On the other hand, as regards the Diptera, the present observation, 
especially in conjunction with Boheman’s statements, particularly 
as to the infestation of Crcadula virescens, Fall. (Thamnotettix sul- 
phurella, Zett.), by the larva of Pipunculus fuscipes, Fall., makes it 
probable that the Diptera of the family Pipunculide are also generally 
parasitic upon Jassidee. 
The Typhlocybe with yellow or whitish elytra form a small group 
of species often living side by side upon the same trees, and resem- 
bling each other so closely that it is almost impossible to distin- 
guish them. Mr. James Edwards, of Norwich, has recently called 
attention to the very distinct differential characters presented by 
the male genital armature in these different species. In accordance 
* Comptes Rendus, July 8, 1889, p. 79; see Annals, supra, p. 254, 
