2. 



D-. J. P. Lotr,;-, ;a of the Jolmn Hoplrin^ Univercity, .''or 

 tlie pri'pose of tletemininr; the orl:'^in and early develonmen-f. 

 of the sporocarp in MarGilla, in thn ^'m^n i.hr,i. t.Vv" some 

 llp;lit mirht he l-h.rovm on tlie question of i' s morpliolO'^ical 

 nativre. It was very soon decided, that tlie he^st vray to reacli 

 tliis end v/ould bo ■''o study in det: il the or^'V^rin arid devel- 

 opment of the leaf also, for comparison, and thus if possiMo 

 to complete the detailed developmental h.istory between th.e 

 leaf motlier cell and tlie sporangium. 



The work has been carried on durin-^ the last t\7o v/interc 

 in the biological laboratory of tlie Jolins Hopkins Univer- 

 sity, under tlie direction of Dr. J. E. Humphrey, io whom for 

 liis cr<>77stant assistance pjid encouraging interest, I wisli 

 here to express my sincere tli^jiks. I am indebted also to 

 Dr. IV. A. Setchell, then of Yale University, for aid in ob- 

 taining material. 



Tlie material used was obtained from Mew Havevi,Conn. an'' 

 Cromwell, Conn. It was fixed in eitlior 95/ alcohol, iX r^irc- 

 mic acid, or a sublimate -acetic mixture ( 5/- of glacial ace- 

 tic acid in • saturated solution of corrosive snblim.ate ). 

 All of tliese gave ,^ood results, but the sublimate mixture 

 was t.he best on tlie wliole, since the cliromic aci^:^ specir-ens 

 did not always stain well and at certain stages of devel- 

 opme^-t f'p .-^Tooliol caused slirinka-^e. Tlie mt^'.terial was stained 



either in toto in Mayer's haemt\lum, or on th.e slide witli 

 this alone or in combination witli Bismark brown, with .^^entir 



