VI CONTENTS. 



CHAPTEK V I 



I'AiK 



Preparation of media Bouillon, gelatin, agar-agar, 

 potato, blood-serum, etc ........ .")9-7(; 



CHAPTEK VII. 



Preparation of the tubes, flasks, etc., in which the media 

 are to be preserved ........ 77-80 



CHAPTER VIII. 



Technique of making plates Esmarch tubes, Petri 

 plates, etc. ......... 81-00 



CHAPTER IX. 



The incubator used in bacteriological work (Jas-piv 

 ure regulator Thermo-regulator The form of burner 

 employed in heating the incubator ..... 91-98 



CHAPTER X. 



The study of colonies Their naked-eye peculiarities 

 and their appearance under different conditions Differ- 

 ences in the structure of colonies from different species of 

 bacteria Stab cultures -Slant cultures .... 99-103 



CHAPTER X I 



Systematic study of an organism Steps necessary in 

 identifying an organism as a definite species . . . 104-121 



CHAPTER XII. 



Methods of staining Solutions employed Preparation 

 and staining of cover-slips Preparation of tissues for 

 section-cutting Staining of tissues Special staining 

 methods .......... 122-150 



CHAPTER XIII. 



Inoculation of animals Subcutaneous inoculation 

 Intra-venous injection ....... 151-158 



