CHAPTER XV. 



Scheme for the complete study of an organism. 



THE following scheme will serve as a guide for the 

 systematic study of au organism : 



1. Its form and grouping as seen when discovered. 



2. Where discovered. 



3. The appearance of its colonies on gelatin plates or 

 Esmarch tubes. 



4. The appearance of its colonies on agar-agar plates 

 or Esmarch tubes. 



5. The appearance of its growth in stab and slant 

 cultures on gelatin. 



6. The appearance of its growth in stab and slant 

 cultures on agar-agar. 



7. Its growth on potato. 



8. Its growth on blood-serum. 



9. Its behavior in bouillon. 



10. Its behavior in milk, plain. 



11. Its behavior in peptone- rosolic-acid solution. 



12. Its behavior in milk containing litmus solution. 



13. What is its normal morphology? What mor- 

 phological changes does it pass through under varying 

 conditions of life? 



14. Does it form spores ? 



15. Is it motile? Are the flagelhe demonstrable by 

 Loffler's method of staining? Is an acid or an alkali 

 to be added to the mordant ; if so, the quantity ? In 



