ANTHKAX INOCULATION IN ANIMALS. 237 



be found dead. Immediately at the point of inoculation 

 but little or no reaction will be noticed, but beyond 

 this, extending for a long distance over the abdomen 

 and thorax, the tissues will be markedly oadematous. 

 Here and there, scattered through this oedematous tissue, 

 small ecchymoses will be seen. The underlying muscles 

 are pale in color. Inspection of the internal viscera 

 reveals no very marked macroscopic changes except in 

 the spleen. This is enlarged, dark in color, soft and 

 brittle. The liver may present the appearance of cloudy 

 swelling ; the lungs may be pale or pale-red in color ; the 

 heart is usually filled with blood. There are no other 

 changes to be seen by the naked eye. Prepare cover- 

 slip preparations from the blood and other viscera. 

 They will all be found to contain the short rods in 

 large numbers. Nowhere can spore-formation be de- 

 tected. Upon microscopic examination of sections of the 

 organs which have been hardened in alcohol, the capilla- 

 ries are seen to be filled with the bacilli ; in some places 

 closely packed together in large numbers, at other points 

 fewer in number. Usually they are present in largest 

 numbers in those tissues having the greatest capillary 

 distribution and at those points at which the circulation is 

 slowest. They are moderately evenly distributed through 

 the spleen. The glomeruli of the kidneys and the capilla- 

 ries of the lungs are frequently quite packed with them. 

 The capillaries of the liver contain them in large numbers. 

 Hemorrhages, probably due to rupture of capillaries by 

 the mechanical pressure of the bacilli which are develop- 

 ing in them, not uncommonly occur. When this occurs 

 in the mucous membranes of the alimentary tract, the 

 blood may escape through the mouth or anus ; when in 

 the kidneys, through the uriniferous tubules. 



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