338 



JACK J. HINMAN, JR., AND MAX LEVINE 



negative in all cases. The reaction to methyl red was slightly alka- 

 line or neutral. Growth was particularly vigorous in this medium 

 and was accompanied by much foaming. 



GROWTH IN SPECIAL MEDIA 



Uric add. There was no evidence of growth in Koser's uric acid 

 medium after 4 days at 37C. 



TABLE 



Lactose bile. Six cultures were inoculated heavily into lactose 

 bile (Difco). No gas was produced in 4 days. 



Gentian violet lactose broth. In lactose broth containing gentian 

 violet in a dilution of 1 : 100,000 there was no gas or other evidence 

 of growth in 5 days at 37C. 



RESISTANCE TO HEAT 



It has previously been mentioned that old cultures taken from 

 lactose agar and milk survived a temperature of 92C. for 20 min- 

 utes although no spores were visible on microscopic examination. 



