Gay and Rusk.] THE LOCUS OF ANTIBODY 



"McGowan: Journal of Pathology and Bacteriology, vol. 14 (1909), 379; 

 vol. 15 (1911), 262. 



"Hektoen and Carlson: Transact. Chicago Path. Soc., vol. S (1909), p. 4; 

 Joura. of Infect. Diseases, vol. 7 (1910), p. 319. 



15 Leuckhardt and Becht: Transact. Chicago Path. Soc., vol. 8 (1911), p. 202. 



"London: Archiv fur biologische Wissenschaften (1901), p. 328. 



17 Jakuschewitsch : Zeit. fur Hygiene, vol. 47 (1904), p. 407. 



18 Brezina: Wein. Klin Wochenschr. (1905), p. 905. 



19 Carrel and Ingebristen: Journ A. M. A., vol. 58 (1911), p. 477. 



"Muller: Central, fur Bakt, I. Abt, Orig., vol. 57 (1911), p. 577. 



21 Hektoen: Transact. Chicago Path. Soc., vol. 8 (1911), p. 138. 



22 Rath: Central, fiir Bakt, vol. 25 (1899), 529. 



23 Weil and Braun: Biochem. Zeitschr., vol. 17 (1910), p. 337. 



24 Kraus and Schiffman: Aunales de 1'Inst. Pasteur, vol. 20 (1906), 225. 

 ^Gruber: Miinch. mediz. Wochenschr. (1897), Nos. 17 and 18. 



26 Ac-hard and Bensaud : Archiv fiir med. Experiment (1896), p. 748. 



27 Wk!al arid Sicard: Annales de 1'Inst. Pasteur, vol 11 (1897), p. 353. 



28 v. Emden: Zeit. far Hygiene, vol. 30 (1899), p. 19. 



"Jatta: Zeit. fur Hygiene, vol. 33 (1900), p. 185. 



30 Girgoleff: Zeit. fiir Imniunitatsforsch, vol. 12 (1912), p. 401. 



81 Petit and Carlson: Jour. Infect. Diseases, vol. 10 (1912), p. 43. 



82 Vaughan, Cumming & McGlumphy: Zeit. fiir Immunitatsforsch., vol. 9 

 (1911), p. 16. 



33 Cantacuzene : Annales de 1'Inst. Pasteur, vol. 22 (1908), p. 54. 



34 Swerew: Rusaky Wratsch. (1910), p. 367; Ref. Jahresbericht der Iminuni- 

 tatsforschung, VI 2 , p. 527. 



36 Hiss and Zinsser: Jour. Med. Research, vol. 19 (1908), p. 399. 



38 Manwaring: Zeitschr. fiir Immunitatsforsch., vol. 8 (1910), p. 1. 



3T Nolf : Archives internat. de Physiolog., Vol. X (1910), p. 37. 



38 Blaizot: Compt. rend. heb. Soc. de Biol., vol. 70 (1911), p. 383. 



38 Gay & Rusk: Univ. Calif. Publ. Path., vol. 2, No. 7 (1912). 



40 Rusk: Univ. Calif. Publ. Path., vol. 2, No. 9 (1912). 



43 Gay and Southard: Jour. Med. Research, vol. 16 (1907), p. 143. 



42 Gay and Rusk : Univ. Calif. Publ. Path., vol. 2, No. 6 (1912). 



48 Bang and Forssmann: Centralblatt fiir Bakt, I. Orig., XL (1905), p. 151. 



"Hintze: Zeit fiir Immunitatsforsch., vol. 6 (1910), p. 113. 



DISCUSSION. 



Dr. W. H. PARK: Observations made on the injection of toxins and 

 antigens into the blood or into the subcutaneous or other tissues in- 

 dicate that for many antibodies several varieties of cells take part. 

 The blood in an animal highly immunized to diphtheria toxin contains 

 about 100 times the quantity of antitoxin as the tissue fluids. If toxin 

 is added to antitoxin, union slowly takes place and the combined toxin 

 is no longer able to excite the production of antitoxin. When a horse 

 having a strongly antitoxic blood is injected intravenously with a 

 definite amount of toxin very little production of antitoxin takes 

 place, because most of it is neutralized by the antitoxin in the blood. 

 The same amount injected in scattered spots subcutaneous!}^ will 

 produce a large amount of antitoxin. The part absorbed into the 

 blood would meet the same fate as that injected intravenously. It 



