complex movements and re sponge to stimuli is n'hen they 

 formed a pirt of the intact org-mism. We are thus led to 

 conclude that altho removal of the nucleus does in some 

 cases interfere with norni.nl 'Movement, at any rate perfect- 

 ly normal movement and irritability are 4uite possible 

 withou*. the presence of the nucleus. 



Closely related to the phenomena of movement are the 

 phenomena of respiration, and there are many interesting 

 observations which bear more or less directly upon the re- 

 lation of the nucleus to eel"! respiration. Aside from the 

 fact that, in aerobic organisms, normal movement implies 

 normal respiration, the activity of the contractile vac- 

 uole indicates that respiration is not interfered with by 

 remov tl of the nucleus. There is a gene^'a4r «greemen% that 

 the function of the contractile vacuole is that of a res- 

 piratory organ, aiding in the removal of carbon dioxide 

 from the interior of such large cells as protozoa. V/hen 

 a protozoon is divided into two parts, the part which 

 lacks a contractile vicuole soon Tovtr^ one, regardless of 

 whether it contains a nucleus or not, Balbiani , Hofer, 

 Stole, Penard). The vacuole contained in the non-nuclear 

 fragment pulsates witl^ about the same frequency as that in 

 the nuclear fragment, but gradually becomes slower with 



(13) 



