the inevitable changes of death (Balbiani, Hofer, Stole ).■;'? 



That the non-nuclear red blood cell respires is a 

 well known fact, and Tashiro (I9l7) has recently demonstrat- 

 ed that the non-nuclear nerve fiber produces carbon dioxide 

 and that this cai-bon dioxide production is increased by 

 stimulation and decreased by anesthetics, .'."oreover, meas- 

 urements of the respiration of nerve ganglia showed that 

 the part of the cell which contained the nucleus produced 

 no more carbon-dioxide than the non-nuclear p-:'rtions, and 

 possibly not as much. 



Loeb ;'i905), however, basing his opinion in part upon 

 the supposed presence in the nucleus of nucleo-proteins 

 which contain iron, suggests "that the nucleus is the organ 

 of oxidation of living matter; and that fragments of cells 

 without a nucleus are not able to regenerate because their 

 oxidative activity has fallen to too low a point'.' But he 

 adds "I do not beleive that without the nucleus all processes 



The regeneration of the contractile vacuole does not, 

 as might at first sight anpear, belie the fact stated 

 above: that regeneration of parts is impossible without 

 the nucleus; for the contractile vacuole is not to be look- 

 ed upon as a structural element of the cell, such as the 

 oral groove, but rather Is its appearance to be regarded as 

 the expression of a certain condition in the protoplasm. 

 The presence of certain granules in the locality where 

 the vacuole has repeatedly appeared (Metcilf , 1910) may 

 be the result rather than the cause of its appearance 

 at this point, for the granules evidently accumulate at 

 the edge of the formed vacuole. 



(14) 



