and a test tube placed over its neck. The cultures v/ere 

 examined d-iily, the amebas washed in sterile water, and 

 transferred to new solutions. 



The hydrolysed casein solution proved to be a fairly 

 good medium in which cases of cell division v/ere occasion- 

 ally observed, but there was never any growth, and the 

 changes which preceded death could be seen in about ten 

 days. The addition of glucose resulted in some improve- 

 ment, but it did not prevent death. 

 Number of amebas living. 



Am.ebas healthy 

 Somewhat abnormal 



Exp. discontinued 



Since this death might be due to the absence of some 



foodstuff, various substances were added to the "diet". 



(40) 



