in hanging drops, side by side, in an Englemann gas-chamher . 

 Nitrogen, which had been washed in 'vater, was then passed 

 thru the chamber, and this atnosphere was maintained until 

 the death of the organisms. In the third exnerinent, the 

 nitrogen was first bubbled from a capillary tube thru 

 strongly alkaline pyrogallic acid, to remove po 3Sible 

 traces of oxyf-en. In the first experiment, the temper- 

 ature of the water jacket surrounding the chamber was 

 raised to 2G''C. in order to accelerate the experiment. 

 The other experiments were performed at 20' C. 



Exp. 1 



Time 



Number of amebas living 

 Nucleated Non-nucleated 



Average life- 



)1j 



(47) 



