Thus there is a distinct diffe^-ence in the suscept- 

 ibility to lack of oxygen in the nucleated and non-nuc- 

 leated cell, but this difference is just ooposite to what 

 v/e should expect if oxidations were proceeding more rapid- 

 ly in the nucleated half. Not only does the non-nucleated 

 cell die more quickly when deprived of oxygen, but it is 

 the first to a^.sume a spherical shape and to cease putting 

 out pseudopods. 



The opposite experiment, that of increasing the supply 

 of oxygen, is also of interest. Were it true that in remov- 

 ing the nuc lexis we have removed the organ of oxidation, and 

 that the cell ia slowly dying of asphyxia, it should be 

 possible to delay the death of the non-nucleated cell by 

 supplying it with more oxygen. On the contrary, it was 

 found that when the nucleated and non-nucleated halves of 

 amebas were kept in an atmosphere of oxygen, both died in 

 less than twelve hours, and the non-nucleated pieces were 

 killed quite as rapidly as those which contained nuclei. 



The experiment was performed in the following 

 manner: Five amebas were cut in half with a fine glass 

 needle, and each half was transferred to a hanging drop 

 in a gas chamber. Oxygen (Linde Air Products) which had 

 been thoroly washed was now passed thru the chamber, and 



f4n) 



