the rapid cooling. 



Thus no difference could be found in the susceptibil- 

 ity of the nuclear and non-nuclear fragments to a rise or 

 fall of temperature. The other method 'vas now employed: 

 keeping ameb ts at different temperatures until death occurred 



Eleven amebas were freed from food particles and debris, 

 and divided into two eiiual parts. Both the nuclear and non- 

 nuclear fragments were kept in a moist chamber at an aver- 

 age room tem.perature of 20" G. 



Number of amebas living 

 Date 

 Lxp.l. 20° C. Nucleated Non-nucleated 



Oct ^ 



Thus, as was shown before, the life of the non-nuclear 

 fragment at ordinary temperature isalmost as long as that 

 of the nuclear fr^agment. 



Ten amebas //ere now divided and each placed in a moist 

 chamber which was kept in a thermostat at 30° C. 



(55) 



