Metabolic Rate in the N on- Nucleated Cell. 



llie rate at which metabolic processes are occurring 

 in different parts of an organism or tissue has been very 

 successfully studied by determining their susceptibility 

 to various poisons, especially to potassium cyanide (Child, 

 1915, 1015 j. Child has brot together a great deal of direct 

 and indirect evidence to show that, in general, regions of 

 high metabolism are more susceptible to poisons than are 

 regions of low metabolism. Accordingly, the susceptibility 

 of the nucleated and non-nucleated cell was investigated 

 with a view to determining how the rate of metabolism was 

 affected by the removal of the nucleus. 



The amebas were cut in half, and one hour after the 



M 

 operation they were transferred to a fresh — •- solution 



50 

 of KGN upon a ho llov;- ground glass slide. The drop of solu- 

 tion was icm.ediately covered with a cover slip to prevent 

 the escape of HCN. Since this could not be entirely pre- 

 vented, the solution gradually became weaker, and as a 



result, those amebas which were not killed in two or three 

 minutes usually lived for more than an hour. Jince small 

 fragments of ameba were found to be more susceptible than 



(56) 



