ual organs. In the great .Majority of Jlorideae the chances 

 are decidedly in favor of finding tetraspores rather than 

 sexual organs . " 



IffiTHODS . 



Of various fixing fluids e.uployed, the v/oalc clirom-acetO' 

 osrnic acid mixture was found to "be oest for cytological de- 

 tails (YA]\tA.TiOUCHI, '06^, p. 425). The time of fixation va- 

 ried from one to ten hours. Paraffin sections 3 and 5 1^ 

 thick v/ere used almost exclusively for the finer details 

 of cell structure, G:."osser anatomical features were foiuid 

 to "he "best made out from mounts in toto . The most success- 

 ful stain employed was Heidenlaain*s iron alum haematoxylin 

 {2 hours in the alum solution, 4 hours in the stain) , fol- 

 lowed "by eosin in QlOve oil, as recomiaended "by Miss Praser 

 (*07). Por some purposes, good differentiation v/as obtain- 

 ed "by following the haematoxylin with gentian violet and 

 extracting the violet with the eosin-clove oil solution. 



Difficulty was experienced in obtaining material show- 

 ing abundant nuclear figures. Plants brought intothe labora- 

 tory and fixed at all hours after leaving been kept in run- 

 ning v/ater showed almost no mitoses. J^avorable ma- 

 dt Ust 



terial was. obtained by fixing in the field at eleven or 

 tv/elve o'clock at night. 



