61 



of about the same si;:e are formed (fig. 119) . These lie in 

 a cliunp in t}ie centre of the nucleus, staining very iieavily 

 with nuclecO' dyes. 



At this stage the tetraspore mother cell rnay "be con- 

 sidered to he ns.ture. The length of a ina.tvire mother cell 

 is about 20 1^, the width 15 A^ , and the diameter of the nu- 

 cleus 7^. rwther changes in tiie mother cell are in an- 

 ticipation of division into tetraspores. 



From tliis time the che.nges in the cytoplasm occto* 

 mainly in connection vitJi the vacuolar area. The vacuoles 

 becoine larger and the whole vacuolaj? area presents a. coarse 

 spongy appearance. In the meshes are deposited numerous 

 spheres of substance staining deeply witli haematoxylin. 

 There is reason to believe that t]iese bodies are derived 

 from the nucleus. As the time of nuclear division approach- 

 es, these granules become larger and fewer in muaber, so 

 that it is possible, by noting their size and nujiiber, to 

 predict in just what stage of mitosis the nucleus v/ill be 

 found. The granules seem to be analogous to the ciiromidial 

 substance of Protozoa (see GOLDS CHI.IIDT, •04) and of some 

 plants (see TISCHLER, '06). 



The cha,nges in the nucleus pjre profound. Host strik- 

 ing is the decrease in staining capacit;' of tiie nucleolar 



