The tetrasporangivun, with the incoaipletely separated 

 spores, increases niarkedl;'- in size. The nuclei also en- 

 large and show abundant chroinatin, in tlie form of the 12- 

 14 masses already mentioned. Each mass is differentiated 

 into lightly and darkly staining areas, "ot infrequently 

 the niunber of these masses is greater thaji this, as many 

 as 20 liaving "been counted in some cases i and sometimes the 

 numher is considerably less than 12. This variability in 



the number of cliromatin masses in the resting nucleus 



e y: fi L D L ^ 

 serves to shov; tiiat they do not flh?~^f-e the same constancy in 



numbers tliat the chromosomes shov/, and therefore are not 



to be relied on as an index of the condition of the nucleus^ 



v.heth.er haploid or diploid. 



As the tetrasporangiiun enltj'ges, t'ne cytoplasm becomes 

 more coaxsely vacuolate, and the vacuoles in the central 

 protoplasmic mass become conspicuous (fig, 14) . The pcU"- 

 titions now grow in until tliey meet in the centre of tlie 

 tetrasporc'-ngilun, their ingrowth being appai'ently aided by 

 the i^osition of the large central vacuoles already mention- 

 ed (fig. 142), The spores are now completely separated, 

 \,it3i the nuclei in tie iuner cornei's. The nuclei v.anaer 

 towciJT'd tlie centre of tlie spores, tJie c]a"'omci.topliores at 

 the same time migrating so as to line tjie entire ierii"!liery 



