84 



The 3-celled. stage is first attained in a'bout 12 hours 

 from the time tlie spoi-e is slied. It persists, under labor- 

 atory conditions, for several days, during which time the 

 three cells change greatly in size, shape, and H.ppear-nce. 

 the ifiost striking changes occur from the second to the 

 third day after the spores ai^e shed. The cells increase 

 greatly in size, and a large central vaxuole is formed in 

 each. The protoplasm a.nd inclusions "being spread over a 

 Itjrger area form a thin filia, in which tlie cliroma.tophores 

 core no longer crowded, hut are separated by considera,ble 

 deer spaces; the sporeling therefore becomes lighter in 

 color and more transpBjr^ent , The following comparison of 

 spoi-elings of the second with those of the third day gives 

 some idea of the grea.t increase in the size of the cells. 



G.^icu.L <-eL(. .'337 'f^^^- -I^S >''-'^- .alcjtr^^^- .073 



bas 0- L ccLL -oHl , /07 .of -073 



totc^L -/2j .7/ 





The number of nuclei in the cells, even after enlarge- 

 ment, is sirif.ll . Several counts indicated that there are 

 in the ajical cell on aii average 25-30 nuclei, in the ioid- 

 fle cell 20-25, in tlie basal cell 5-10. These nuclei in 



