12 THE VENOMS OF CERTAIN THANATOPHIDEJ2. 



The precipitate thus obtained is washed as in the preparation of the water- 

 venom-globulin, and when thus purified it does not give any color reaction with the 

 ammonia or the ferrocyanide and acetic-acid tests for copper, and therefore cannot 

 be regarded as a salt of this metal. 



The copper-venom-globulin gives the following reactions: 



Decided reactions with the usual proteid tests. 



Sodic chloride (0 75 per cent.) insoluble. 

 (10 " ) insoluble. 



the addition of crystals of sodic chloride seems to dissolve it 

 slightly; this solution is cleared somewhat by boiling; the 

 same eifect by boiling the suspended mixture; the clearing 

 is no doubt the result of the formation of coagula. 



Carbonic acid insoluble. 



Sodic carbonate very soluble, forming a beautiful clear solution ; boiling has no effect; the solu- 

 tion is precipitated by carbonic acid. 



Hydrochloric acid (0.4 per cent.) exceedingly soluble. 



Metaphosphoric acid insoluble ; boiling no effect. 



Orthophosphoric acid very soluble, forming an absolutely clear solution; boiling has no decided 



effect. 



Sodic metaphospliate insoluble ; boiling no effect. 



Sodic orthophosphate soluble in a much larger amount than is necessary in dissolving the water- 

 venom-globulin ; boiling has no effect, unless to clear the 

 solution some. 



Poiassic sulphate insoluble ; boiling no effect. 



Calcic chloride less soluble than waier-venom-globulin. 



Acetic acid (5 per cent.) very soluble. 



Acetic acid (glacial) very soluble. 



The Dialysis-venom-globulin. The filtrate, after the separation of the watcr- 

 venom-globulin and copper-venom-globulin, still gives a decided amount of coagula 

 by boiling, and also all of the characteristic color reactions for proteids. If the 

 filtrate be now subjected to dialysis, best by means of a large dialyser placed over 

 running water, in the course of twenty-four hours a considerable amount of pre- 

 cipitate will be deposited within the dialyser, and which may be collected on a 

 filter, and repeatedly washed as in the preparation of the preceding globulins. 



If dialysis is carried on for a sufficient length of time the whole of this principle 

 will be precipitated, since the filtrate from the globulin will give no coagula by 

 boiling, nor any precipitate by strong nitric acid. A proteid still remains in solu- 

 tion, however, which has been already alluded to as being a peptone. This body 

 being less dialysable than the salts which hold the globulins in solution, still remains 

 in part within the dialyser, even when the salts are so fully withdrawn as to entirely 

 precipitate the globulins. 



The dialysis-venom-globulin gives the following reactions: 



Decided reactions with the usual proteid tests. 

 Sodic chloride (0.75 per cent.) insoluble. 



(10 " ) slightly soluble. 



(crystals') more soluble, forming a very cloudy solution ; boiling clears the 

 solution some; the same degree of clearing does not occur in 

 the mixture without the sodic chloride. 



the addition of carbonic acid to the solution with crystals causes 

 a beautiful clear solution, which is made cloudy by boiling. 



