18 



THE VENOMS OF CERTAIN T H A N A T P H I D E 



by dialysing venom for forty-eight hours. The dialysate was perfectly clear and 

 neutral in reaction: 



Boiling no result until after a few moments, when it becomes cloudy, the cloudiness increasing 



as boiling continues ; strong nitric acid dissolves the precipitate. 



Color reactions for proteidt- the xantho-proteic, Millon and Biuret reactions are all obtained. 

 Ferric chloride no effect. 

 Cupric sulphate no effect. 

 Mercuric chloride no effect. 

 Mercuric nitrate decided precipitate. 

 Absolute alcohol no precipitate. 



Potassic ferrocyanide -(- weak acetic acid precipitate. 

 Nitric acid (strong) no precipitate. 

 Hydrochloric acid (strong) no precipitate. 

 Acetic acid (strong) no precipitate. 



Sodic chloride (saturation) precipitate; acetic acid, large quantity, dissolves. 

 Potassic hydrate to saturation precipitate. 

 Tannic acid decided precipitate. 

 Basic acetate of lead decided precipitate. 



Several very remarkable facts are the coagulation by prolonged boiling and the 

 non-precipitation by mercuric chloride and absolute alcohol. Since this peptone 

 is precipitated by weak acetic acid in the presence of potassic ferrocyanide it has 

 a slight resemblance to Meissner's A peptone, although materially differing, as 

 some of the above reactions show, from any other described body of this class. 



As a matter of some interest, it is desirable to know if similar globulins in 

 different venoms are identical in their chemical nature, or whether they give any 

 reactions which may distinguish them. We have accordingly, as in previous cases, 

 placed the reactions of the corresponding globulins side by side. 



I. Water-venom-globulin. 



