43 



Material of this species for distillation was obtained from the following 

 localities in N.S.W. Lismore, 23rd July, 1900 ; Tumbulgum, i5th . September, 

 1900; Bulahdelah, 3rd September, 1900; and Barber's Creek, I7th March, 1899. 

 The oils from all this material were practically identical in all their characters, 

 as can be seen from the following table : 



The fractions, from those oils redistilled, were identical in each case, so that 

 the results show a remarkable constancy with the several samples. The oil from 

 Bulahdelah, for instance, when compared with that obtained nearly two months 

 earlier from Lismore, is worthy of note. The amount of oil distilling below 167 C., 

 the rotations and specific gravities of the several fractions, the saponification 

 number for the esters, together with the solubility in alcohol, all go to show this 

 close agreement. The nitrosochloride was prepared with the pinene, and this 

 melted at, 103-104 C. Although the oil of this form of E. saligna contains so 

 much pinene, yet it scarcely falls into the group insoluble in 10 volumes of 80 

 per cent, alcohol ; this peculiarity must, therefore, be due to the esters, and to 

 the free alcohol, because the amount of cineol present is too small to influence 

 the solubility. The crude oils of all our samples formed clear solutions with 

 either 7, 8, or 9 volumes 80 per cent, alcohol. 



To determine the approximate amount of free alcohol in the oil of this 

 species a portion of the Lismore oil was acetylated in the usual way, carefully 

 freed from acid and saponified. The saponification number had then increased 

 to 56-04, so that the amount of alcohols present is represented by the saponifi- 

 cation number 27-14. The esterised oil had a distinct odour of amyl-acetate. 

 Although amyl-alcohol was present in combination as well as in the free condition, 

 yet, it may be that a portion was also in combination with the valeric acid as an 

 ester. If this is so, then the amyl-alcohol previously determined in the oil of 

 E. globulus might have been derived from a similar ester. 



The mixed oils of the above tabulated samples were kept in the dark, and 

 in October, 1919, or nineteen years afterwards, the sample was again analysed. 

 But little alteration had taken place during all that time, thus again illustrating 

 the great stability of the pinene Eucalyptus oils. The specific gravity of the 

 crude oil at 15 C. was 0-8950. On rectification 36 per cent, distilled below 160, 

 76 per cent, below 180, and 82 per cent, below 190. 



The portion distilling be'ow 190 C. had specific gravity at 15 = 0-8749; 

 rotation a D + 30-8, and refractive index at 20 = 1-4652. 



