275 



. The cineol, determined by the phosphoric acid method in the first fraction, 

 was 25 per cent. (O.M.), indicating about 20 per cent, in the original oil. 



It was from this species that the first Eucalyptus oil was obtained. This 

 is referred to in White's " Journal of a Voyage to New South Wales, 1790," p. 266. 



Material for distillation from trees that are considered to be E. piperita, 

 which have urn-shaped fruits resembling in miniature those characteristic of 

 E. corymbosa, was obtained from Wingello, N.S.W., in January, 1901. These 

 trees grow extensively in that neighbourhood. The oil had resemblances to that 

 of the type, but contained considerably more phellandrene ; pinene was present, 

 and eudesmol also detected. Cineol was present to the extent of about 10 to 15 

 per cent, in the crude oil. 



The crude oil had specific gravity at 15 C. = 0-8785 ; rotation D - - 35-5 ; 

 refractive index at 20 = 1-4772, and was insoluble in 10 volumes 80 per cent, 

 alcohol. The saponification number for the esters and free acid was 2-8. 



On rectification, 2 per cent, distilled below 167 C. (corr.). Between 

 167-183, 83 per cent, distilled; between 183-225, 7 per cent, came over, and 

 between 225-260, 6 per cent, distilled. These fractions gave the following 

 results : 



First fraction, sp. gr. at 15 C. = 0-8700; rotation D 39'3- 

 Second ,, ,, =0-8877; ,, -29-4. 



Third ,, ,, ,, = 0-9298; ,, not taken. 



The cineol did not appear to exceed 10 or 15 per cent, in the crude oil, 

 consequently the phosphoric acid method was not suitable for quantitative 

 determination. 



From the rotation figures it is apparent that the activity was due to the 

 phellandrene present, but the whole results indicate that this form of E. piperita 

 is closely associated with the type. 



This sample from Wingello had been stored in the dark, and in September, 

 1919, was again analysed. Very little alteration had taken place during the 

 eighteen years the oil had been kept. 85 per cent, distilled below 190 C. 



The crude oil had sp. gr. at 15 C. == 0-8828; rotation D - - 30-4 ; refrac- 

 tive index at_2o = 1-4778. 



The rectified portion =0-8720; rotation a D --37-7; refrac- 

 tive index at 20 = 1-4744. 



The cineol was determined by the resorcinol method in the rectified portion ; 

 the result was 27 per cent., but as 5 per cent, of piperitone was also present, that 

 amount had to be deducted, thus 22 per cent, of cineol is the corrected amount. 



