Almond integuments (prepared as above). H. No. 1 gave a dense, black 

 mycelium and abundant conidia. 



Corn meal (moistened and autodaved in test-tubes}. H. No. 1 gave 

 dense, black borders and greenish-black interstices. 



Corn-starch (prepared as above}. H. No. 1 gave a dense,_black, even 

 surface-growth with little or no aerial mycelium. 



SUMMARY CONCERNING GROWTH ON RICE AND SIMILAR 

 SUBSTANCES 



With Helminthosporium, as with Fusarium, rice tubes are of value for 

 differentiating species. The important constituent seems to be in the 

 aleurone layer. Pearl-barley has similar and different, though less, value. 

 Whole wheat grains, whole rice, beans, etc., do not give these color reactions. 



Color phenomena in fungi have been discussed by Smith (106), and 

 Hedgecock (65), and by Stewart and Hodgkiss (119), who cite several 

 papers bearing upon the subject. These, however, deal mainly with 

 conditions of acidity and general carbohydrates rather than with proteid 

 relations.* 



MISCELLANEOUS VEGETABLE MEDIA 



Parts (plugs) of various vegetables were placed in test-tubes in 

 some cases with glass slips with about 2 c.c. of water and autoclaved. 

 The chief resulting characters of various Helminthosporium plantings 

 on such media consisted in the development of aerial mycelium and 

 the pellicle over the surface of the water. 



Potato plugs (prepared in the usual way). H. No. 1 gave on these 

 plugs large development of woolly, white aerial mycelium. At the line 

 of contact with the glass the growth was dense and black. No sclerotia 

 developed in four days. This medium possessed little differential value, 

 only H. Nos. 11, 12, and 17 showing clear differences. 



Potato plugs on glass slips. The slips were placed in test-tubes (in 

 the manner shown in Fig. 2, page 95), and then slices of potato, which were 

 so cut that they could barely be crowded into the tubes. They were then 

 autoclaved. This gave opportunity for observation at three places: (1) the 

 exposed potato surface; (2) the contact of the potato with the glass slip and 

 with the wall of the tube; and (3) the border at the edge of the contact 

 (cf. with terms under rice, p. 86). H. No. 1 here gave a dense black 



*In this connection see paragraph on carbohydrates on p ^.ge 100. 



