EXPERIMENTAL WORK. 163 



date much faith), the truth came out : rather than disappoint me, 

 they said, they had put a divot in one of the smaller tributaries of 

 the loch (a divot is a cut turf) and lifted out some spawning trout 

 of their very redds, the poachers I Some of the eggs I spawned 

 into a dish, and then milted, and others I spawned into the dishes, 

 after having first milted the males into them. I also tried the 

 following experiments. It will be convenient to follow out the 

 experiments separately as far as recorded in my notes. 



Experiment 1. 



Before leaving Sauchie I milted a small burn-trout into a test- 

 tube ; this was at 9 A.M. At 1.20 P.M. I used the milt on some eggs 

 from a Loch Leven trout; I placed 143 eggs in some sphagnum 

 moss, and laid them down in a slate tray at 11 P.M. same evening. 



Experiment 2. 



I had also taken with me from Sauchie 3 male burn-trout 

 alive ; these I milted on some Loch Leven eggs taken dry at 

 1.30 P.M., and packed in moss and laid down at Sauchie at 11 P.M. 

 Total, 200. 



Experiment 3. 



I took a small male, which I had brought from home with the 

 others, and milted him on a few Loch Leven eggs ; the quantity of 

 milt was very small, and the male did not weigh two ounces, the 

 object being to see how little milt would be sufficient. This 

 experiment also I packed in moss. Total, 160. 



Experiment 4. 



On the following day I took some milt I had bottled at Loch 

 Leven, and, stripping a burn-trout which I had not fully stripped 

 on the 21st, milted the ova; the milt had been twenty hours in 

 the small bottle. Total, 15 ova. 



Experiment 5. 



I took 50 Loch Leven eggs, which I had carried in a carefully 

 dried and tightly corked tube, and milted with plenty of milt 



