CHAPTER X 



EXPERIMENTS WITH NUCLEIN THE LOWERING OF THE 



COEFFICIENT OF DIFFUSION CAUSED BY EXTRACTS 



OF DEAD HAEMAL GLAND DIVISIONS INDUCED IN 



LYMPHOCYTES FOR THE FIRST TIME REVELATIONS 



CONCERNING THESE DIVISIONS THE ROLES PLAYED 



BY THE ALTMANN'S GRANULES, NUCLEI, AND 

 NUCLEOLI IN THEIR CELL-DIVISION 



BEFORE proceeding to discuss the problem of the 

 causation of cell-division, it is necessary to state that 

 another piece of information was at our disposal which 

 we believed to be intimately associated with cell-division, 

 although the fact was not appreciated when the point 

 was first noticed. During experimentation with a 

 mixture of stain and alkaloid on blood-cells it was 

 noticed that with a citrated mixture of Unna's stain 

 and the alkaloid atrbpine the lymphocytes sometimes 

 extrude granules (fig. 39) from their cell- walls. These 

 granules remain attached to the cell by means of a 

 streamer, apparently derived from the cell-wall itself. 

 The extrusion appears to be a deliberate one on the 

 part of the cell, and the granule ultimately becomes 

 separated from it altogether. This extrusion, or " flagel- 

 lation" as we erroneously called it, has been confirmed 



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