AUXETICS ISOLATED 315 



divisions in lymphocytes in the experimental ten 

 minutes. 



Amount to be contained in the 10 cc. of jelly.. 



Dried extract of Testis . . . 0.025 gramme 



" Pancreas . . . 0.025 



" Muscle . . . 0.025 



" Spleen . . . 0.01 



" Liver . . . 0.002 



Experimentally all these extracts were employed in a. 

 5-per-cent solution. Divisions in lymphocytes were in- 

 duced with the first three by adding . 5 cc. of the solution 

 to the 5 cc. of coefficient jelly, together with 0.7 cc. of 

 1-per-cent solution of atropine sulphate, 1 cc. of the 

 5-per-cent solution of sodium bicarbonate, and made up 

 to a total of 10 cc. with 2.8 cc. of water. The jellies 

 were boiled and films made from them in the usual way. 

 It is obvious, therefore, that all the extracts contain 

 some substance or substances which cause cell-division 

 in lymphocytes and in leucocytes. To induce these 

 divisions on the microscope slide in the experimental ten 

 minutes, it is necessary to augment the action of the ex- 

 tracts with atropine. Suprarenal extract, however, evi- 

 dently containing more of the active substance than the 

 others, will induce divisions without any augmenting sub- 

 stance. Putrefaction will augment the power of the ex- 

 tracts like the alkaloid, and it was presumed that, this 

 putrefaction had this effect through the presence of the 

 alkaloids of putrefaction. This point, however, was not 

 investigated till afterwards, as we were immediately 

 concerned in finding out if possible what the agents 

 were in these extracts of dead tissues which cause the 

 division of white blood-corpuscles. 



