EXPERIMENTS WITH GLOBIN 331 



It must be understood that if the jelly on which the 

 cells are resting contains . 02 gramme, or more, of 

 globin, the red cells become distorted and the white 

 cells are killed without divisions being induced in 

 them. 



We think that it should be mentioned that it is 

 quite within the realms of possibility that the malaria 

 parasite proliferated in response to the active agent 

 contained in globin; but although we have tried a few 

 experiments to endeavour to prove the point, we have 

 not succeeded in determining it. Malarial crescents 

 frequently flagellate in any case within ten minutes of 

 their being shed; and although we have mixed the shed 

 blood containing them with citrated solutions of globin, 

 it has been impossible for us to satisfy ourselves that 

 the flagellation has been accelerated by its action. In 

 the cases of malaria at our disposal there have not been 

 a very large number of parasites in the blood, and time 

 was lost during the experiments in finding them. 

 Hence we cannot speak definitely on this point, but 

 it was the consideration of the life-history of the malaria 

 parasite which \vas the chief factor which led us to 

 investigate the auxetic property of globin; and there is 

 no doubt whatever that globin contains some auxetic, 

 although it is not so powerful as that contained in 

 suprarenal extract. 



Globin contains no kreatin so far as we can ascer- 

 tain, and the solution of globin which we have used is 

 free of haematin, as proved by spectroscope examina- 

 tion, and there are only traces of lipochrome. What 

 the exact nature of the auxetic substance contained in 



